The Buzz Surrounding Cannabix Technologies Inc. (CSE: BLO) (OTC: BLOZF) (2024)

Cannabix Technologies Inc. (CSE: BLO) (OTC PINK: BLOZF) (Frankfurt: 8CT) (the “Company or Cannabix”) developer of marijuana breathalyzer devices for law enforcement and the workplace reports that it will be presenting at the International Association for Chemical Testing (IACT) conference in April. Cannabix’s Dr. Bruce Goldberger Ph.D., will be presenting onScience & Technology Behind Cannabis Breath Testingand the Company’s marijuana breathalyzer system at the conference workshop session. IACT is composed of governmental agency personnel involved in chemical testing related to traffic safety. IACT members are scientific and criminal justice professionals, including forensic scientists, regulatory inspectors, and law enforcement personnel with representatives from a majority of states and international members. IACT has standing committees to address various areas including legislation, training, new equipment and procedures and manufacturers of breath testing equipment.

TheCannabis Breath Testingworkshop at IACT 2024 is designed to provide background information regarding the pharmaco*kinetics and pharmacodynamics of cannabinoids in breath, present the current technology for collection devices from commercial vendors, and discuss the necessary laboratory workflows and analytical considerations when testing these samples.

Dr. Bruce Goldberger is a professor and the former Chief of the Forensic Medicine Division in the Department of Pathology, Immunology and Laboratory Medicine in the College of Medicine at the University of Florida. Dr. Goldberger served as Director of the University of Florida forensic toxicology laboratory for 30 years. Dr. Goldberger is the Principal Investigator of the Florida Drug-Related Outcomes Surveillance and Tracking System and Co-investigator of the National Drug Early Warning System. Dr. Goldberger is a consultant for the Centers for Disease Control and Prevention’s National Center for Injury Prevention and Control, Overdose Mortality Team, and the National Center for Health Statistics, Division of Vital Statistics. Dr. Goldberger is a consultant and senior advisor to Cannabix Technologies Inc.

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Cannabix Technologies Inc. (CSE: BLO) (OTC PINK: BLOZF) (Frankfurt: 8CT) (the “Company or Cannabix”) developer of marijuana and alcohol breath testing devices reports that it has shipped its Industrial Series Contactless Alcohol Breathalyzers (“CAB” devices) for certification testing and is rebranding the “CAB” name under the new “Breath Logix” product series. The Breath Logix product series is part of the Company’s portfolio of marijuana and alcohol screening devices.

The Breath LogixIndustrial SeriesAlcohol breath detection device is an autonomous wall mounted unit that is ruggedized and designed for the workplace (Figure 1). A breath sample can be delivered either by straw (Figure 2) or in contactless mode. These devices have been delivered to globally recognized certification laboratory, CE cert GmbH in Germany, for independent alcohol standards testing which is a step required before sales of the device can begin. The regulatory process for certification of alcohol breath detection devices is well established, and will aid in an analogous process for the Company’s marijuana (delta-9 THC) breathalyzer products.

Figure 1. Breath Logix Industrial Series Alcohol Testing System

Figure 2. Breath Logix Industrial Series in use with straw. Contactless breath test mode also available.

Breath Logix Industrial Series Features

The Breath Logix Industrial Series is a weather resistant device with a host of requested features including patent pending pre-calibrated cartridge technology (See Figure 3) which will allow site safety administrators to easily maintain their devices, and eliminate the need for costly calibration equipment and time-consuming site visits from technicians for maintenance. This device can be used for pre-access alcohol testing, random testing, start-of-shift testing, pre-employment testing, return-to-work testing and post-incident testing. The device is currently being piloted as a solution for the 24/7 sobriety program which exists in several states.

The Breath Logix Alcohol device automatically checks the sobriety of a user and can take a picture to confirm and record identity while a breath sample is being delivered. Upon detection of positive breath alcohol result, the device will deliver a precise Blood Alcohol Content (BAC) level on the screen, and send a real-time alert via text message, e-mail and to a dedicated web portal. Furthermore, the system logs user BAC for incident reporting and historical investigations. The Breath Logix helps organizations save money by deploying an autonomous alcohol screening device which eliminates the need for dedicated alcohol screening administrators using conventional handheld devices.

Figure 3. Breath Logix pre-calibrated alcohol sensor cartridge technology

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Cannabix Technologies Inc. (CSE: BLO) (OTC PINK: BLOZF) (the “Company or Cannabix”) developer of marijuana and alcohol breath testing devices is pleased to report that Dr. Phillip Olla (Ph.D.) has joined the board of directors. Dr. Olla is an expert in breath technologies, breathomics, healthcare innovation, artificial and medical informatics. Dr. Olla has served as the CEO of Audacia Bioscience, a health informatics company who performed substantial work in cannabis breath research and development. Dr. Olla is one of only a handful of scientists globally who has ever conducted delta-9 THC analysis in blood and breath. Dr. Olla is an associate professor at University of Detroit Mercy in Michigan and is the Vice Chair of the MedHealth Consortium.

Dr. Olla has established himself as a sought-after global speaker. He has authored seven books and more than 60 scholarly publications, showcasing his expertise in technology innovation and healthcare. He worked in the telecom and technology sectors for over a decade, working for British Telecom and O2 and Oracle. His contributions within the digital health arena have been acknowledged internationally, exemplified by his invitation to speak at a United Nations conference and World Health Organization. Notably, his professional journey includes collaborations with companies like IBM, Roche Diagnostics and Morgan Stanley. Dr. Olla holds a Ph.D. in Management Information Systems (MIS) from Brunel University, United Kingdom.

Dr. Olla’s contributions to “breathomics” are particularly noteworthy. His published research in this domain has established him as a leader in understanding the complex interplay of breath components and their potential applications in medical diagnostics.

Rav Mlait, CEO, stated, “Dr. Olla’s expertise in multi-modal breath analysis will aid in advancing Cannabix’s marijuana and alcohol breathalyzer technologies in the U.S. and Europe. Dr. Olla has amassed a considerable network of industry professionals and experience that will help Cannabix move to commercialization. Dr. Olla will also help explore additional applications and market opportunities for Cannabix’s novel breath testing technologies.”

Stock Options

The Company reports the cancellation of 1,500,000 stock options as follows: 450,000 exercisable at $0.80, 375,000 exercisable at $0.50, 500,000 exercisable at $0.55, 175,000 exercisable at $0.35.

The Company is granting a total of 2.7M incentive stock options to directors and consultants of the Company. 2,225,000 options will be exercisable at $0.25 per share for five years. 475,000 stock options will be exercisable at $0.25 per share for 2 years, and subject to vesting provisions.

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Cannabix Technologies Inc. (CSE: BLO) (OTC PINK: BLOZF) (the “Company or Cannabix”) developer of marijuana and alcohol breath testing devices reports that analysis results from the collection of breath samples from a recent “green lab” testing day in Montgomery County, Maryland were successfully analyzed (with delta9-THC detected and quantified from breath samples after smoking and edibles consumption) at a third-party location for the first time. Breath samples taken on January 3, 2024 during the Marylandgreen labheld by the Montgomery County Police Department were shipped to the North Louisiana Criminalistics Laboratory (“NLCL”) (Fig. 1 and Fig. 2), based in Shreveport, LA for analysis. This represents the first time, such quantification analysis, with human breath samples, collected and analyzed with Cannabix’s proprietary laboratory-based marijuana detection equipment, has been successfully conducted outside of Cannabix’s own labs – marking a critical step to show reproducibility of Cannabix hardware and analysis methods in multiple and independent locations.

The data collected from 11 volunteer subjects (10 smokers and 1 subject who consumed THC infused edibles) showed similar results to those achieved during past green lab studies. The baseline (prior to consumption) delta9-THC breath concentrations were in most cases very low indicating no recent use of cannabis products. Thirty (30) minutes after observed consumption, the delta9-THC concentrations in breath per cartridge increased significantly – with the highest recorded values being around 25 ng/cartridge. After 60 minutes the THC concentrations in breath decreased but remained above baseline values. Subjects were then asked to re-dose after approximately 80 minutes after first cannabis consumption and analysis showed elevated levels of delta9-THC in breath in later time points, after re-dosing, even when compared to the levels observed after initial consumption.

Samples were analysed roughly 48 hours after collection and no obvious adverse effects related to storing or shipping were observed during the analysis process. The calibration curve acquired prior to analysing samples at the NLCL lab was similar to those used at the Company’s Burnaby, BC lab location.

Rav Mlait, CEO of Cannabix stated, “This is significant milestone for our marijuana breathalyzer system as we have now been able duplicate our latest quantification processes – outside of our own laboratories – at NCLC. Administrators at NCLC were able to prepare their calibration curve and duplicate Cannabix’s quantification analysis methods and achieve quantified breath samples for delta-9 THC from smoking and edibles. This marks a major step showing the ability to reproduce of our breath sample testing methods in multiple locations.”

The Company’s handheld Breath Collection Unit (“BCU”) (Fig. 3) and newly developed laboratory “MS Breath Sampler” (Fig. 4) are being used together to provide a new method for drug detection that complements gold-standard mass spectrometry (MS) and significantly simplifies laboratory analysis methods, reduces sample turnaround times (thus minimizing operating costs), while maintaining sensitive, precise results. By comparison, existing and legacy breath and saliva testing procedures require several sample extraction and preparation steps prior to analysis, and analysis itself can take from 1-3 hours per sample. This is expensive and impractical. Also, existing and legacy breath and saliva testing procedures tend to have inefficient, time-consuming collection methods, and recoveries are still often poor. Cannabix instrumentation is designed to be easy-to-use and seamlessly interfaced with techniques that toxicologists are already familiar with, requiring minimal training by all users.

Cannabix continues to test and develop its BCU and MS Breath Sampler to quantify delta-9 THC in human breath samples. Cannabix scientists are using an internal standard to generate a calibration curve for THC quantification in breath samples. Currently, a limit of detection and limit of quantification have been achieved with human subjects in the picogram range. This allows detection of THC from smoking and edibles up to 4+ hours after consumption.

Fig. 1 Cannabix hosted at North Louisiana Crime Lab

Fig. 2 Cannabix MSBS at North Louisiana Crime Lab

Fig. 3 Cannabix updated Breath Collection Unit (BCU)

Fig. 4 Cannabix “MS Breath Sampler” technology coupled with Thermo TSQ Ultra

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